Skip to main content

Articles

Page 35 of 35

  1. Homology-dependent selective degradation of RNA, or post-transcriptional gene silencing (PTGS), is involved in several biological phenomena, including adaptative defense mechanisms against plant viruses. Small...

    Authors: Tiziana Pandolfini, Barbara Molesini, Linda Avesani, Angelo Spena and Annalisa Polverari
    Citation: BMC Biotechnology 2003 3:7
    Content type: Research article Published on:

  2. DNA microarrays are now routinely used to monitor the transcript levels of thousands of genes simultaneously. However, the array fabrication method, hybridization conditions, and oligodeoxyribonucleotide probe...

    Authors: David R Dorris, Allen Nguyen, Linn Gieser, Randall Lockner, Anna Lublinsky, Marcus Patterson, Edward Touma, Timothy J Sendera, Robert Elghanian and Abhijit Mazumder
    Citation: BMC Biotechnology 2003 3:6
    Content type: Research article Published on:

  5. Double-stranded RNA (dsRNA) is a potent initiator of gene silencing in a diverse group of organisms that includes plants, Caenorhabditis elegans, Drosophila and mammals. We have previously shown and patented that...

    Authors: Francisco Tenllado, Belén Martínez-García, Marisol Vargas and José Ramón Díaz-Ruíz
    Citation: BMC Biotechnology 2003 3:3
    Content type: Research article Published on:

  6. Bacterial artificial chromosomes (BACs) have been used extensively for sequencing the human and mouse genomes and are thus readily available for most genes. The large size of BACs means that they can generally...

    Authors: Christine Magin-Lachmann, George Kotzamanis, Leonardo D'Aiuto, Ernst Wagner and Clare Huxley
    Citation: BMC Biotechnology 2003 3:2
    Content type: Research article Published on:

  7. The ability to manipulate the genetic networks underlying the physiological and behavioural repertoires of the adult honeybee worker (Apis mellifera) is likely to deepen our understanding of issues such as learni...

    Authors: Gro V Amdam, Zilá LP Simões, Karina R Guidugli, Kari Norberg and Stig W Omholt
    Citation: BMC Biotechnology 2003 3:1
    Content type: Methodology article Published on:

  8. Allosteric ribozymes (aptazymes) that have extraordinary activation parameters have been generated in vitro by design and selection. For example, hammerhead and ligase ribozymes that are activated by small organi...

    Authors: Kristin M Thompson, Heather A Syrett, Scott M Knudsen and Andrew D Ellington
    Citation: BMC Biotechnology 2002 2:21
    Content type: Research article Published on:

  9. When generating transformed plants, a first step in their characterization is to obtain, for each new line, an estimate of how many copies of the transgene have been integrated in the plant genome because this...

    Authors: Giovanna Mason, Paolo Provero, Anna Maria Vaira and Gian Paolo Accotto
    Citation: BMC Biotechnology 2002 2:20
    Content type: Methodology article Published on:

  10. Ostrich (Struthio camelus) breeds have been gaining increasing significance around the world. The large-scale sex determination of chicks is an important task in the development of these breeds. To date, two PCR-...

    Authors: Wilson Malagó Jr, Heitor M Franco, Euclides Matheucci Jr, Adriana Medaglia and Flavio Henrique-Silva
    Citation: BMC Biotechnology 2002 2:19
    Content type: Methodology article Published on:

  11. Efficient transformation and regeneration methods are a priority for successful application of genetic engineering to vegetative propagated plants such as grape. The current methods for the production of trans...

    Authors: Bruno Mezzetti, Tiziana Pandolfini, Oriano Navacchi and Lucia Landi
    Citation: BMC Biotechnology 2002 2:18
    Content type: Research article Published on:

  12. The brine shrimp lethality assay is considered a useful tool for preliminary assessment of toxicity. It has also been suggested for screening pharmacological activities in plant extracts. However, we think tha...

    Authors: José Luis Carballo, Zaira L Hernández-Inda, Pilar Pérez and María D García-Grávalos
    Citation: BMC Biotechnology 2002 2:17
    Content type: Methodology article Published on:

  13. Hep27 monoclonal (Hep27 Mab) is an antibody against hepatocellular carcinoma. Hep27 Mab itself can inhibit the growth of a hepatocellular carcinoma cell line (HCC-S102). We attempted to produce a single-chain ...

    Authors: Duanpen Sandee, Sumalee Tungpradabkul, Manae Tsukio, Tadayuki Imanaka and Masahiro Takagi
    Citation: BMC Biotechnology 2002 2:16
    Content type: Research article Published on:

  14. The ability of transfected synthetic small interfering (si) RNAs to suppress the expression of specific transcripts has proved a useful technique to probe gene function in mammalian cells. However, high produc...

    Authors: Eric Devroe and Pamela A Silver
    Citation: BMC Biotechnology 2002 2:15
    Content type: Methodology article Published on:

  15. The power of DNA microarrays derives from their ability to monitor the expression levels of many genes in parallel. One of the limitations of such powerful analytical tools is the inability to detect certain t...

    Authors: Allen Nguyen, Connie Zhao, David Dorris and Abhijit Mazumder
    Citation: BMC Biotechnology 2002 2:14
    Content type: Research article Published on:

  16. In a variant of the standard PCR reaction termed bridging, or jumping, PCR the primer-bound sequences are originally on separate template molecules. Bridging can occur if, and only if, the templates contain a ...

    Authors: Shumo Liu, David S Thaler and Albert Libchaber
    Citation: BMC Biotechnology 2002 2:13
    Content type: Research article Published on:

  17. Cationic lipid DNA complexes based on DOTAP (1,2-dioleoyl-3-(trimethyammonium) propane) and mixtures of DOTAP and cholesterol (DC) have been previously optimized for transfection efficiency in the absence of s...

    Authors: Godwin Nchinda, Klaus Überla and Olaf Zschörnig
    Citation: BMC Biotechnology 2002 2:12
    Content type: Research article Published on:

  18. Non-invasive autofluorescent reporters have revolutionized lineage labeling in an array of different organisms. In recent years green fluorescent protein (GFP) from the bioluminescent jellyfish Aequoria Victoria ...

    Authors: Anna-Katerina Hadjantonakis, Suzanne Macmaster and Andras Nagy
    Citation: BMC Biotechnology 2002 2:11
    Content type: Methodology article Published on:

  19. This study tested a low-volume (20–30 μl/20–30 μg DNA) jet injection method for intradermal delivery of a DNA vaccine. Jet injection offers the advantages of a needle-less system, low-cost, rapid preparation o...

    Authors: Shuxun Ren, Minglin Li, Joanne M Smith, Louis J DeTolla and Priscilla A Furth
    Citation: BMC Biotechnology 2002 2:10
    Content type: Methodology article Published on:

  20. We investigated the encapsulation mechanism of enzymes into liposomes. The existing protocols to achieve high encapsulation efficiencies are basically optimized for chemically stable molecules. Enzymes, howeve...

    Authors: Jacques-Philippe Colletier, Barnabé Chaize, Mathias Winterhalter and Didier Fournier
    Citation: BMC Biotechnology 2002 2:9
    Content type: Methodology article Published on:

  21. Determination of clot lysis times on whole blood, diluted whole blood, plasma or plasma fraction has been used for many years to assess the overall activity of the fibrinolytic system. We designed a completely...

    Authors: K Zouaoui Boudjeltia, Ph Cauchie, Cl Remacle, M Guillaume, D Brohée, JL Hubert and M Vanhaeverbeek
    Citation: BMC Biotechnology 2002 2:8
    Content type: Methodology article Published on:

  24. Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limi...

    Authors: Keejong Chang, Jin Qian, MeiSheng Jiang, Yi-Hsin Liu, Ming-Che Wu, Chi-Dar Chen, Chao-Kuen Lai, Hsin-Lung Lo, Chin-Ton Hsiao, Lucy Brown, James Bolen Jr, Hsiao-I Huang, Pei-Yu Ho, Ping Yao Shih, Chen-Wen Yao, Wey-Jinq Lin…
    Citation: BMC Biotechnology 2002 2:5
    Content type: Research article Published on:

  25. Parthenocarpy, or fruit development in the absence of fertilization, has been genetically engineered in eggplant and in other horticultural species by using the DefH9-iaaM gene. The iaaM gene codes for tryptophan...

    Authors: Nazzareno Acciarri, Federico Restaino, Gabriele Vitelli, Domenico Perrone, Michela Zottini, Tiziana Pandolfini, Angelo Spena and Giuseppe Leonardo Rotino
    Citation: BMC Biotechnology 2002 2:4
    Content type: Research article Published on:

  26. The suitability of the strain Rhodococcus erythropolis ATCC 25544 grown in a two-liter fermentor as a source of cholesterol oxidase has been investigated. The strain produces both cell-linked and extracellular ch...

    Authors: Mar M Sojo, Roque R Bru and Francisco F García-Carmona
    Citation: BMC Biotechnology 2002 2:3
    Content type: Research article Published on:

  27. Agrobacterium vitis is a causal agent of crown-gall disease. Trifolitoxin (TFX) is a peptide antibiotic active only against members of a specific group of α-proteobacteria that includes Agrobacterium and its clos...

    Authors: Thomas C Herlache and Eric W Triplett
    Citation: BMC Biotechnology 2002 2:2
    Content type: Research article Published on:

  28. Genetic engineering of parthenocarpy confers to horticultural plants the ability to produce fruits under environmental conditions that curtail fruit productivity and quality. The DefH9-iaaM transgene, whose predi...

    Authors: Tiziana Pandolfini, Giuseppe L Rotino, Serena Camerini, Roberto Defez and Angelo Spena
    Citation: BMC Biotechnology 2002 2:1
    Content type: Methodology article Published on:

  29. The DNA-dependent RNA polymerase from T7 bacteriophage (T7 RNAP) has been extensively characterized, and like other phage RNA polymerases it is highly specific for its promoter. A combined in vitro / in vivo sele...

    Authors: Jijumon Chelliserrykattil, George Cai and Andrew D Ellington
    Citation: BMC Biotechnology 2001 1:13
    Content type: Research article Published on:

  30. Transgenic mice have been used extensively to analyze gene function. Unfortunately, traditional transgenic procedures have only limited use in analyzing alleles that cause lethality because lines of founder mi...

    Authors: Ravi P Misra, Sarah K Bronson, Qi Xiao, Wendy Garrison, Jixuan Li, Roong Zhao and Stephen A Duncan
    Citation: BMC Biotechnology 2001 1:12
    Content type: Methodology article Published on:

  31. Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and ...

    Authors: Larry R Karns PhD, Anne Kisielewski BSc, Kathryn M Gulding BSc and Dan Theodorescu MD PhD
    Citation: BMC Biotechnology 2001 1:11
    Content type: Research article Published on:

  32. To maintain populations of microbial cells under controlled conditions of growth and environment for an indefinite duration is a prerequisite for experimentally evolving natural isolates of wild-type species o...

    Authors: Valérie A de Crécy-Lagard, Jacques Bellalou, Rupert Mutzel and Philippe Marlière
    Citation: BMC Biotechnology 2001 1:10
    Content type: Methodology article Published on:

  36. Modern drug discovery is concerned with identification and validation of novel protein targets from among the 30,000 genes or more postulated to be present in the human genome. While protein-protein interactio...

    Authors: Renuka C Pillutla, Ku-chuan Hsiao, Renee Brissette, Paul S Eder, Tony Giordano, Paul W Fletcher, Michael Lennick, Arthur J Blume and Neil I Goldstein
    Citation: BMC Biotechnology 2001 1:6
    Content type: Research article Published on:

  37. There have been many attempts to develop new materials with stability and high affinity towards immunoglobulins. Some of glycolipids such as gangliosides exhibit a high affinity toward immunoglobulins. However...

    Authors: Jae Hong Im, Takashi Nakane, Hiroshi Yanagishita, Toru Ikegami and Dai Kitamoto
    Citation: BMC Biotechnology 2001 1:5
    Content type: Research article Published on:

  38. Hydrophobic polystyrene is the most common material for solid phase immunoassay. Proteins are immobilized on polystyrene by passive adsorption, which often causes considerable denaturation. Biological macromol...

    Authors: Vadim V Shmanai, Tamara A Nikolayeva, Ludmila G Vinokurova and Anatoli A Litoshka
    Citation: BMC Biotechnology 2001 1:4
    Content type: Research article Published on:

  40. The Gavacâ„¢ vaccine against the cattle tick Boophilus microplus has proven its efficacy in a large number of controlled and field experiments. However, this vaccine could be further improved by searching for new a...

    Authors: Manuel Rodríguez Valle, Carlos Montero, Héctor Machado and Marisdania Joglar
    Citation: BMC Biotechnology 2001 1:2
    Content type: Research article Published on:

  41. Infections of bacterial cultures by bacteriophages are serious problems in biotechnological laboratories. Apart from such infections, prophage induction in the host cells may also be dangerous. Escherichia coli i...

    Authors: Agata Czyz, Marcin Los, Borys Wrobel and Grzegorz Wegrzyn
    Citation: BMC Biotechnology 2001 1:1
    Content type: Methodology article Published on:
Submit manuscript

Follow

Annual Journal Metrics

  • 2022 Citation Impact
    3.5 - 2-year Impact Factor
    3.5 - 5-year Impact Factor
    0.880 - SNIP (Source Normalized Impact per Paper)
    0.654 - SJR (SCImago Journal Rank)

    2023 Speed
    10 days submission to first editorial decision for all manuscripts (Median)
    155 days submission to accept (Median)

    2023 Usage 
    1,134,875 downloads
    518 Altmetric mentions 

BMC Biotechnology

ISSN: 1472-6750

Contact us