Table 1 Strains , plasmids used in this study
From: A novel MVA-mediated pathway for isoprene production in engineered E. coli
Name | Relevant characteristics | References |
|---|---|---|
Strains | ||
E.coli BL21(DE3) | F− ompT hsdSB (rB −mB −) gal dcm rne131 λ(DE3) | Invitrogen |
E.coli DH5α | deoR, recA1, endA1, hsdR17(rk-,mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 | Takara |
YJM30 | E.coli BL21(DE3)/pYJM30 | This study |
YJM31 | E.coli BL21(DE3)/pYJM32 | This study |
YJM32 | E.coli BL21(DE3)/pYJM33,pYJM16 | This study |
YJM33 | E.coli BL21(DE3)/pYJM34, pYJM16 | This study |
YJM34 | E.coli BL21(DE3)/ pYJM35, pYJM16 | This study |
Plasmids | ||
pACYCDuet-1 | P15A ori, lacI T7lac, Cmr | Novagen |
pCOLADuet-1 | ColA ori, lacI T7lac, Kanr | Novagen |
pBAD 18 | pBR322 ori, araBAD, Ampr | [29] |
pET-28a(+) | pBR322 ori, lacI T7lac, Kanr | Novagen |
pYJM16 | pACYCDuet-1 carrying mvaE and mvaS from Enterococcus faecalis | [9] |
pYJM30 | pCOLADuet-1 carrying oleT JE from Jeotgalicoccus species | This study |
pYJM31 | pBAD18 carrying oleT JE from Jeotgalicoccus species | This study |
pYJM32 | pCOLADuet-1 carrying ohyA EM from Elizabethkingia meningoseptica | This study |
pYJM33 | pCOLADuet-1 carrying oleT JE from Jeotgalicoccus species and ohyA EM from Elizabethkingia meningoseptica | This study |
pYJM34 | pET-28a(+) carrying oleT JE from Jeotgalicoccus species and ohyA EM from Elizabethkingia meningoseptica | This study |
pYJM35 | pBAD18 carrying oleT JE from Jeotgalicoccus species and ohyA EM from Elizabethkingia meningoseptica | This study |