Fig. 4
From: A novel MVA-mediated pathway for isoprene production in engineered E. coli
Optimization of the expression levels of oleT JE and ohyA EM . The expression of oleT JE aand ohyA EM under the control of T7 promoter (YJM32) achieved much higher isoprene production than when the gene were under the control of the araBAD promoter (YJM34). The strain YJM33 using a high copy number plasmid (pET-28a(+)) achieved the highest isoprene production (52.2 μg/L). The experiment was conducted in triplicate