Fig. 3From: A novel MVA-mediated pathway for isoprene production in engineered E. coli Enzymatic assay for isoprene production by OhyAEM using GC-MS and SDS-PAGE analysis. a GC-MS analysis of a isoprene sample produced by the OhyAEM assay mixtures. b SDS-PAGE analysis of OhyAEM. CK: cell lysate from BL21(DE3) containing pCOLADuet-1. 1: crude cell extracts from YJM31. 2: purified OhyAEM Back to article page