Fig. 2From: A novel MVA-mediated pathway for isoprene production in engineered E. coli Enzymatic assay for 3-methyl-3-buten-1-ol production by OleTJE using GC-MS and SDS-PAGE analysis. a GC-MS analysis of a 3-methyl-3-buten-1-ol sample produced by the OleTJE assay mixtures. b SDS-PAGE analysis of OleTJE. CK: cell lysate from BL21(DE3) containing pCOLADuet-1. 1: crude cell extracts from YJM30. 2: purified OleTJE Back to article page