Fig. 1
From: A novel MVA-mediated pathway for isoprene production in engineered E. coli
Production of isoprene via the novel MVA-mediated pathway used in this study. The dashed box shows the traditional pathway for MVA conversion to isoprene [8, 9], while the new pathway constructed in this study is within the solid-lined box. Enzyme symbols: MvaE (acetyl-CoA acetyltransferase/HMG-CoA reductase) and MvaS (HMG-CoA synthase) from Enterococcus faecalis; ERG12 (mevalonate kinase), ERG8 (phosphomevalonate kinase), ERG19 (mevalonate pyrophosphate decarboxylase) and IDI1 (IPP isomerase) from Saccharomyces cerevisiae; IspS (isoprene synthase) from Populus alba. OleTJE from Jeotgalicoccus species; OhyAEM from Elizabethkingia meningoseptica. Pathway intermediates: A-CoA, acetyl-CoA; AA-CoA, acetoacetyl-CoA; HMG-CoA, hydroxymethylglutaryl-CoA; Mev-P, mevalonate 5-phosphate; Mev-PP, mevalonate pyrophosphate. IPP, isopentenyl pyrophosphate; DMAPP, dimethylallyl pyrophosphate