Articles

Interference chromatography: a novel approach to optimizing chromatographic selectivity and separation performance for virus purification

Oncolytic viruses are playing an increasingly important role in cancer immunotherapy applications. Given the preclinical and clinical efficacy of these virus-based therapeutics, there is a need for fast, simpl...

Authors: Lisa A. Santry, Renaud Jacquemart, Melissa Vandersluis, Mochao Zhao, Jake M. Domm, Thomas M. McAusland, Xiaojiao Shang, Pierre M. Major, James G. Stout and Sarah K. Wootton

Colorectal cysts as a validating tool for CAR therapy

Treatment of cancers has largely benefited from the development of immunotherapy. In particular, Chimeric Antigen Receptor (CAR) redirected T cells have demonstrated impressive efficacy against B-cell malignan...

Authors: Pierre Dillard, Maren Lie, Elizabeth Baken, Viola Hélène Lobert, Emmanuelle Benard, Hakan Köksal, Else Marit Inderberg and Sébastien Wälchli

In vitro biosynthesis of Ag, Au and Te-containing nanostructures by Exiguobacterium cell-free extracts

The bacterial genus Exiguobacterium includes several species that inhabit environments with a wide range of temperature, salinity, and pH. This is why the microorganisms from this genus are known generically as p...

Authors: Javier Orizola, Mirtha Ríos-Silva, Claudia Muñoz-Villagrán, Esteban Vargas, Claudio Vásquez and Felipe Arenas

Recombinant PAL/PilE/FlaA DNA vaccine provides protective immunity against Legionella pneumophila in BALB/c mice

Legionella pneumophila (L.pneumophila), a Gram-negative small microorganism, causes hospital-acquired pneumonia especially in immunocompromised patients. Vaccination may be an effective method for preventing L.pn...

Authors: Yingying Chen, Zehui Yang, Ying Dong and Yu Chen

Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications

Environmental pollution is one of the major problems that the world is facing today. Several approaches have been taken, from physical and chemical methods to biotechnological strategies (e.g. the use of oxido...

Authors: Wissal Ben Ali, Delphine Chaduli, David Navarro, Christian Lechat, Annick Turbé-Doan, Emmanuel Bertrand, Craig B. Faulds, Giuliano Sciara, Laurence Lesage-Meessen, Eric Record and Tahar Mechichi

GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system

Recombinant protein production and purification of large protein complexes in eukaryotes requires efficient methods to generate multi-gene expression constructs, where each individual gene is under the control...

Authors: Jana Neuhold, Katharina Radakovics, Anita Lehner, Florian Weissmann, Maria Queralt Garcia, Mari Carmen Romero, Nicholas S. Berrow and Peggy Stolt-Bergner

Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation

Gene editing using the CRISPR/Cas9 system has become a routinely applied method in several plant species. The most convenient gene delivery system is Agrobacterium-mediated gene transfer with antibiotic selection...

Authors: Zsófia Bánfalvi, Edina Csákvári, Vanda Villányi and Mihály Kondrák

Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli

Advantages of translocation of recombinant proteins to the periplasm in Escherichia coli include simplified downstream processing, and improved folding and in vivo activity of the target protein. There are, howev...

Authors: Agnieszka Gawin, Helga Ertesvåg, Sine Alise Hartvigsen Hansen, Jostein Malmo and Trygve Brautaset

Retraction Note: DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA

An amendment to this paper has been published and can be accessed via the original article.

Authors: Zhen Jia, Yueyi Shi, Lei Zhang, Yipeng Ren, Tong Wang, Lejun Xing, Baorong Zhang, Guolan Gao and Rongfa Bu

Utilization of egg-laying hens (Gallus Gallus domesticus) for production of ingredients for human consumption and animal feed

In Norway, 3 million discarded egg-laying hens are destructed annually, which equals 1500 tons pure hen meat. Due to the slaughter methods used, this raw material is handled as a high-risk waste, while in real...

Authors: Veronica Hjellnes, Rasa Šližyte, Turid Rustad, Ana Karina Carvajal and Kirsti Greiff

Process optimization and effect of thermal, alkaline, H₂O₂ oxidation and combination pretreatment of sewage sludge on solubilization and anaerobic digestion

This study investigated the feasibility of enhancing anaerobic digestion of sewage sludge with triple, dual, and individual pretreatment of waste activated sludge with heat, alkalinity, and hydrogen peroxide. ...

Authors: Salar Siami, Behnoush Aminzadeh, Razieh Karimi and Seyed Mostafa Hallaji

Phytoremediation effect of Medicago sativa colonized by Piriformospora indica in the phenanthrene and cadmium co-contaminated soil

The coexistence of polycyclic aromatic hydrocarbons (PAHs) and heavy metals has deleterious effects on environmental quality. Few reports have studied the mechanisms of plant inoculation with Piriformospora indic...

Authors: Liang Li, Pengyue Zhu, Xiaoyang Wang and Zhenhua Zhang

Generation of an engineered food-grade Lactococcus lactis strain for production of an antimicrobial peptide: in vitro and in silico evaluation

Foodborne pathogens and their biofilms are considered as one of the most serious problems in human health and food industry. Moreover, safety of foods is a main global concern because of the increasing use of ...

Authors: Abbas Tanhaeian, Mehdi Mirzaii, Zana Pirkhezranian and Mohammad Hadi Sekhavati

Lentivirus expressing shRNAs inhibit the replication of contagious ecthyma virus by targeting DNA polymerase gene

Contagious ecthyma or Orf is known as a zoonotic disease remains prevalently worldwide despite the application of some control strategies against it. RNAi particularly shRNA provides us with the chance to tack...

Authors: Leila Asadi Samani, Behnaz Saffar, Azam Mokhtari and Ehsan Arefian

A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression

Cell-free protein expression has become a widely used alternative of in vivo, cell-based systems in functional and structural studies of proteins. The wheat germ-based method outstands from the commercially av...

Authors: Szilvia Krisztina Nagy, Brigitta Margit Kállai, Judit András and Tamás Mészáros

Development and biological activity of long-acting recombinant human interferon-α2b

The type I human interferon (IFN) family consists of a group of cytokines with a multiplicity of biological activities, including antiviral, antitumor, and immunomodulatory effects. However, because the half-l...

Authors: Qian Zhang, Chao Wang, Fenlian Ma, Lihong Yao, Hanchun Gao, Luyan Zhu and Lishu Zheng

Optimised production of an anti-fungal antibody in Solanaceae hairy roots to develop new formulations against Candida albicans

Infections caused by fungi are often refractory to conventional therapies and urgently require the development of novel options, such as immunotherapy. To produce therapeutic antibodies, a plant-based expressi...

Authors: Marcello Catellani, Chiara Lico, Mauro Cerasi, Silvia Massa, Carla Bromuro, Antonella Torosantucci, Eugenio Benvenuto and Cristina Capodicasa

An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea

Botryosphaeria dothidea causes apple white rot and infects many tree plants. Genome data for B. dothidea are available and many pathogenesis-related genes have been predicted. However, a gene manipulation method ...

Authors: Bao-Zhu Dong and Li-Yun Guo

Cassava/peanut intercropping improves soil quality via rhizospheric microbes increased available nitrogen contents

Intercropping, an essential cultivation pattern in modern agricultural systems, increases crop yields and soil quality. Cassava and peanut intercropping systems exhibit advantages in solar utilization and cadm...

Authors: Xiumei Tang, Ruichun Zhong, Jing Jiang, Liangqiong He, Zhipeng Huang, Guoying Shi, Haining Wu, Jing Liu, Faqian Xiong, Zhuqiang Han, Ronghua Tang and Longfei He

A snapshot of microbial diversity and function in an undisturbed sugarcane bagasse pile

Sugarcane bagasse is a major source of lignocellulosic biomass, yet its economic potential is not fully realised. To add value to bagasse, processing is needed to gain access to the embodied recalcitrant bioma...

Authors: Leigh Gebbie, Tuan Tu Dam, Rebecca Ainscough, Robin Palfreyman, Li Cao, Mark Harrison, Ian O’Hara and Robert Speight

Integration, abundance, and transmission of mutations and transgenes in a series of CRISPR/Cas9 soybean lines

As with many plant species, current genome editing strategies in soybean are initiated by stably transforming a gene that encodes an engineered nuclease into the genome. Expression of the transgene results in ...

Authors: Jean-Michel Michno, Kamaldeep Virdi, Adrian O. Stec, Junqi Liu, Xiaobo Wang, Yer Xiong and Robert M. Stupar

Soft - sensing modeling based on ABC - MLSSVM inversion for marine low - temperature alkaline protease MP fermentation process

Aiming at the characteristics of nonlinear, multi-parameter, strong coupling and difficulty in direct on-line measurement of key biological parameters of marine low-temperature protease fermentation process, a...

Authors: Bo Wang, Meifang Yu, Xianglin Zhu and Li Zhu

Trauma induced tissue survival in vitro with a muscle-biomaterial based osteogenic organoid system: a proof of concept study

The translation from animal research into the clinical environment remains problematic, as animal systems do not adequately replicate the human in vivo environment. Bioreactors have emerged as a good alternati...

Authors: Tao He, Jörg Hausdorf, Yan Chevalier and Roland M. Klar

Generation of recombinant baculovirus expressing atoxic C-terminal CPA toxin of Clostridium perfringens and production of specific antibodies

Clostridium perfringens is the causative agent of several diseases and enteric infections in animals and humans. The virulence of C. perfringens is largely attributable to the production of numerous toxins; of th...

Authors: Katia Forti, Monica Cagiola, Martina Pellegrini, Lucia Anzalone, Antonella Di Paolo, Sara Corneli, Giulio Severi and Antonio De Giuseppe

A self-aggregating peptide: implications for the development of thermostable vaccine candidates

The use of biomaterials has been expanded to improve the characteristics of vaccines. Recently we have identified that the peptide PH_(1–110) from polyhedrin self-aggregates and incorporates foreign proteins to fo...

Authors: Adolfo Cruz-Reséndiz, Jesús Zepeda-Cervantes, Alicia Sampieri, Carlos Bastián-Eugenio, Gonzalo Acero, J. Iván Sánchez-Betancourt, Goar Gevorkian and Luis Vaca

Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein

Trichophyton rubrum is an obligate human parasitic fungus and responsible for approximately 80–90% of dermatomycosis in human. Molecular genetic manipulations of this pathogen are challenging and available tools ...

Authors: Oliver Blechert, Huan Mei, Xiaohui Zang, Hailin Zheng, Guanzhao Liang and Weida Liu

Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway. Several studies have demonstrated t...

Authors: Gabriel Levin, Bruna Andrade Aguiar Koga, Gustavo Gross Belchior, Ana Claudia Oliveira Carreira and Mari Cleide Sogayar

Construction and characterization of a high-quality cDNA library of Cymbidium faberi suitable for yeast one- and two-hybrid assays

Cymbidium faberi is one of the oldest cultivars of oriental orchids, with an elegant flower fragrance. In order to investigate the molecular mechanism and the functions of related proteins in the methyl jasmonate...

Authors: Yanqin Xu, Junjiang Zhou, Qingqing Liu, Kunpeng Li and Yin Zhou

Intein-mediated recombinant expression of monomeric B22Asp desB30 insulin

Insulin controls hyperglycemia caused by diabetes, and virtually all treatments require exogenous insulin. However, the product’s extensive post-translational modifications have hindered the manufacture of rec...

Authors: Minmin Zhang, Yunlong Zhang, Bingnan Wu, Yanhao Peng, Altaf Ahmed Simair, Geoffery W. Siegel, Changrui Lu and Ting Chen

Screening of cellulolytic bacteria from rotten wood of Qinling (China) for biomass degradation and cloning of cellulases from Bacillus methylotrophicus

Cellulosic biomass degradation still needs to be paid more attentions as bioenergy is the most likely to replace fossil energy in the future, and more evaluable cellulolytic bacteria isolation will lay a found...

Authors: Lingling Ma, Yingying Lu, Hong Yan, Xin Wang, Yanglei Yi, Yuanyuan Shan, Bianfang Liu, Yuan Zhou and Xin Lü

Development and applications of a monoclonal antibody against caprine interferon-gamma

Interferon-gamma (IFN-γ) is an important mediator of type I immune response and has antiviral, immunoregulatory and anti-tumor properties, plays a wide range of roles in inflammation and autoimmune diseases. T...

Authors: Wen-Tao Ma, Qi Liu, Meng-Xia Ning, Yu-Xu Qi, Saad Rehman and De-Kun Chen

Optimized production of a biologically active Clostridium perfringens glycosyl hydrolase phage endolysin PlyCP41 in plants using virus-based systemic expression

Clostridium perfringens, a gram-positive, anaerobic, rod-shaped bacterium, is the third leading cause of human foodborne bacterial disease and a cause of necrotic enteritis in poultry. It is controlled using anti...

Authors: Rosemarie W. Hammond, Steven M. Swift, Juli A. Foster-Frey, Natalia Y. Kovalskaya and David M. Donovan

The next generation of biopanning: next gen sequencing improves analysis of bacterial display libraries

Bacterial surface display libraries are a popular tool for novel ligand discovery due to their ease of manipulation and rapid growth rates. These libraries typically express a scaffold protein embedded within ...

Authors: Sarah D. Stellwagen, Deborah A. Sarkes, Bryn L. Adams, Mia A. Hunt, Rebecca L. Renberg, Margaret M. Hurley and Dimitra N. Stratis-Cullum

Fecal DNA isolation and degradation in clam Cyclina sinensis: noninvasive DNA isolation for conservation and genetic assessment

To avoid destructive sampling for conservation and genetic assessment, we isolated the DNA of clam Cyclina sinensis from their feces. DNA electrophoresis and PCR amplification were used to determine the quality o...

Authors: Min Zhang, Min Wei, Zhiguo Dong, Haibao Duan, Shuang Mao, Senlei Feng, Wenqian Li, Zepeng Sun, Jiawei Li, Kanglu Yan, Hao Liu, Xueping Meng and Hongxing Ge

Medfly-Wolbachia symbiosis: genotype x genotype interactions determine host’s life history traits under mass rearing conditions

Wolbachia pipientis is a widespread, obligatory intracellular and maternally inherited bacterium, that induces a wide range of reproductive alterations to its hosts. Cytoplasmic Incompatibility (CI) is causing em...

Authors: Georgios A. Kyritsis, Antonios A. Augustinos, Ioannis Livadaras, Carlos Cáceres, Kostas Bourtzis and Nikos T. Papadopoulos

A walk on the wild side: gut bacteria fed to mass-reared larvae of Queensland fruit fly [Bactrocera tryoni (Froggatt)] influence development

The Queensland fruit fly, Bactrocera tryoni (Froggatt) (Diptera, Tephritidae) is the most significant insect pest of Australian horticulture. Bactrocera tryoni is controlled using a range of tools including the S...

Authors: Lucas Alexander Shuttleworth, Mohammed Abul Monjur Khan, Terrence Osborne, Damian Collins, Mukesh Srivastava and Olivia Louise Reynolds

Potential of a fly gut microbiota incorporated gel-based larval diet for rearing Bactrocera dorsalis (Hendel)

The Oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), is an important polyphagous pest of horticultural produce. The sterile insect technique (SIT) is a proven control method against many i...

Authors: Mahfuza Khan, Kajla Seheli, Md. Abdul Bari, Nahida Sultana, Shakil Ahmed Khan, Khandokar Fahmida Sultana and Md. Anwar Hossain

Horizontal transfer and finalization of a reliable detection method for the olive fruit fly endosymbiont, Candidatus Erwinia dacicola

The olive fly, Bactrocera oleae, is the most important insect pest in olive production, causing economic damage to olive crops worldwide. In addition to extensive research on B. oleae control methods, scientists ...

Authors: Gaia Bigiotti, Roberta Pastorelli, Roberto Guidi, Antonio Belcari and Patrizia Sacchetti

The host fruit amplifies mutualistic interaction between Ceratitis capitata larvae and associated bacteria

The Mediterranean fruit fly Ceratitis capitata is a major pest in horticulture. The development of fly larvae is mediated by bacterial decay in the fruit tissue. Despite the importance of bacteria on larval devel...

Authors: Doron Shalom Yishai Zaada, Michael Ben-Yosef, Boaz Yuval and Edouard Jurkevitch

Olive fruit fly rearing procedures affect the vertical transmission of the bacterial symbiont Candidatus Erwinia dacicola

The symbiosis between the olive fruit fly, Bactrocera oleae, and Candidatus Erwinia dacicola has been demonstrated as essential for the fly’s larval development and adult physiology. The mass rearing of the olive...

Authors: Patrizia Sacchetti, Roberta Pastorelli, Gaia Bigiotti, Roberto Guidi, Sara Ruschioni, Carlo Viti and Antonio Belcari

Biochemical and nutritional characterization of the medfly gut symbiont Enterobacter sp. AA26 for its use as probiotics in sterile insect technique applications

Enterobacter sp. AA26 was recently isolated from the midgut of Ceratitis capitata (Wiedemann) and it was shown to have positive effects in rearing efficiency when used as larval probiotics. In this study, biomass...

Authors: Konstantinos Azis, Ioanna Zerva, Paraschos Melidis, Carlos Caceres, Kostas Bourtzis and Spyridon Ntougias

The oesophageal diverticulum of Dirioxa pornia studied through micro-CT scan, dissection and SEM studies

Dirioxa pornia (Diptera, Tephritidae) (Island fly) is an Australian native species related to a number of pestiferous fruit flies but, unlike many of the pest species, has not been studied extensively due to its ...

Authors: Kala Bhandari, Peter Crisp and Michael A. Keller

The effect of diet and radiation on the bacterial symbiome of the melon fly, Zeugodacus cucurbitae (Coquillett)

Symbiotic bacteria contribute to a multitude of important biological functions such as nutrition and reproduction and affect multiple physiological factors like fitness and longevity in their insect hosts. The...

Authors: Elias D. Asimakis, Mahfuza Khan, Panagiota Stathopoulou, Carlos Caceres, Kostas Bourtzis and George Tsiamis

Simultaneous hydrolysis with lipase and fermentation of rapeseed cake for iturin A production by Bacillus amyloliquefaciens CX-20

Rapeseed cake (RSC), as the intermediate by-product of oil extraction from the seeds of Brassica napus, can be converted into rapeseed meal (RSM) by solvent extraction to remove oil. However, compared with RSM, R...

Authors: Wenchao Chen, Xuan Li, Xuli Ma, Shouwen Chen, Yanping Kang, Minmin Yang, Fenghong Huang and Xia Wan

Morphological analysis of Apolipoprotein E binding to Aβ Amyloid using a combination of Surface Plasmon Resonance, Immunogold Labeling and Scanning Electron Microscopy

Immunogold labeling in combination with transmission electron microscopy analysis is a technique frequently used to correlate high-resolution morphology studies with detailed information regarding localization...

Authors: Tohidul Islam, Anna L. Gharibyan, Cheng Choo Lee and Anders Olofsson

A new method for quantitative detection of Lactobacillus casei based on casx gene and its application

The traditional method of bacterial identification based on 16S rRNA is a widely used and very effective detection method, but this method still has some deficiencies, especially in the identification of close...

Authors: Xiaoyang Pang, Ziyang Jia, Jing Lu, Shuwen Zhang, Cai Zhang, Min Zhang and Jiaping Lv

Purification of the recombinant green fluorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography

The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensiv...

Authors: Jie Dong, Xiangzhen Ding and Sheng Wang

Improvement and use of CRISPR/Cas9 to engineer a sperm-marking strain for the invasive fruit pest Drosophila suzukii

The invasive fruit pest Drosophila suzukii was reported for the first time in Europe and the USA in 2008 and has spread since then. The adoption of type II clustered regularly interspaced short palindromic repeat...

Authors: Hassan M. M. Ahmed, Luisa Hildebrand and Ernst A. Wimmer

13th Annual Biotechnology Congress (BAC Madrid 2019): abstract collection

Authors:

Tuning the performance of CAR T cell immunotherapies

Simultaneous advances in gene editing, T cell engineering and biotechnology currently provide an opportunity for rapid progress in medicine. The approval of chimeric antigen receptor (CAR) T cell therapies by ...

Authors: Noah H. Richardson, Jordan B. Luttrell, Jonathan S. Bryant, Damian Chamberlain, Saleem Khawaja, Indira Neeli and Marko Radic