Table 1 Fidelity metrics and estimated error rates for Neq2X and Neq2X7
FC50 all templates [μM] | Error rate estimated according to de Paz [bp-1] | Literature / Manufacturer reference [bp-1] | |
|---|---|---|---|
Neq2X | 2.6×10-5 | 1.3×10-8 | 6.3×10-5 [25] |
Neq2X7 | 2.9×10-4 | 1.8×10-6 | |
Taq | 3.0×10-4 | 2.0×10-6 | 2.2×10-5 [37] |
Phusion | 4.4×10-7 [38] | ||
Error rate improvement compared to Taq | |||
Template | Neq2X | Neq2X7 | |
All templates | 155 | 1.1 | |
TA | 177 | 139 | |
TT | 285 | 5.2 | |
TC | 289 | 0.2 | |
TG | 5.5 | 0.01 | |