Fig. 3

Determination of Neq2X7 fidelity (A) Schematic of the MagNIFI assay principle inspired by de Paz et al. B Frequency of mismatches observed at the error enrichment site for all four polymerases, measured across four different templates (n = 8). A nonlinear response curve was fitted to the data obtained from Neq2X, Neq2X7, and Taq samples. C Concentration of rare dNTP resulting in a 50% error frequency (FC₅₀) obtained from a nonlinear fit to the error rate measurements based on the four individual templates. The geometric mean with a 95% confidence interval is shown underlying the individual data points. D, E Error frequency measurements and response curve fit resolved by the individual templates for Neq2X7, Neq2X, and Taq. Data points represent the mean, the error bars show the standard deviation of two biological replicates