Fig. 2

PCR comparison of the DNA polymerases performance for different templates, amplification times, and conditions using 1 pmol of each DNA polymerase enzyme run on a 1% agarose gel. A Three different amplicons with sizes 3000, 6000, and 12,000 bp were produced using 1 min of elongation per kilobase. For each template, two conditions were evaluated: (i) the amplification of DNA with a mixture of dNTPs containing dTTP (dATP, dTTP, dCTP, and dGTP), and (ii) dUTP replacing dTTP (dATP, dUTP, dCTP, and dGTP). B The same templates as in panel A were amplified using 15 s of elongation time per kilobase. C High GC content template amplification from three different genomic DNA regions of Streptomyces were amplified by PCR. From left to right, 73% GC of 1540 bp expected size, 76.6% GC of 1680 bp expected size, and 66.3% GC of 1516 bp expected size. D Fast-PCR amplification of a 630 bp template. P (PfuX7), N (Neq2X), N7 (Neq2X7), and Ph (Phusion) DNA polymerases. (M) DNA marker in base pairs. For PCR conditions, primers, and templates, see the Methods section