Table 3 Reagents utilized in the modified high-salt low-pH method, high-salt method, and NaOH low-salt method

Modified high-salt low-pH method

Buffer A

1.25 M NaCl, 0.25 M Ascorbic acid, 10 mM Sodium metabisulfite, 50 mM Tris [tris (hydroxymethyl) aminomethane hydrochloride] -HCl (pH 8.0), 7 mM EDTA (ethylene diamine tetraacetic acid), 1% w/v PVP (polyvinylpyrrolidone)-40, 0.1% w/v BSA (bovine serum albumin), 1 mM DTT (dithiothreitol)

Buffer B

1.25 M NaCl, 50 mM Tris-HCl (pH 8.0), 25 mM EDTA (pH 8.0), 1% w/v PVP-40, 0.1% w/v BSA, 1 mM DTT

Buffer C

50 mM Tris-HCl, 25 mM EDTA (pH 8.0), 1.25 M NaCl, 2.0% SDS (sodium dodecyl sulfate)

High-salt method

Buffer D

1.25 M NaCl, 50 mM Tris-HCl (pH 8.0), 5 mM EDTA, 0.1% w/v BSA, 0.1% v/v β-mercaptoethanol

NaOH low-salt method

Buffer E

0.5 M NaOH, 7 mM EDTA, 1 g PVP-40, 0.2% v/v β-mercaptoethanol

Buffer F

0.62 M NaCl, 50 mM Tris-HCl, 7 mM EDTA, 2 g PVP-40, 1.5 mM DTT

Buffer G

0.35 M Sorbitol, 50 mM Tris-HCl, 25 mM EDTA