Fig. 3
From: Deep orange gene editing triggers temperature-sensitive lethal phenotypes in Ceratitis capitata
CRISPR strains obtained after targeting Ccdor via CRISPR HDR. The Ccdor reference sequence (Ccap 3.2.1 (accession GCA_905071925.1)) with the sgRNA target sequence in grey and the PAM site is shown in the first row. The rows below represent the three mutant strains obtained via CRISPR HDR with the SNP responsible for the E839K mutation (dor E839K), the 12Â bp deletion (dor 12del), and the 51Â bp duplication inserted via false integration of the HDR repair template (dor 51dup), respectively. The nucleotide change responsible for the E839K mutation is shown in red, while the duplication is underlined