Fig. 1
From: Deep orange gene editing triggers temperature-sensitive lethal phenotypes in Ceratitis capitata
A Schematic representation of the Ccdor gene spanning six exons. Pep3/Vps18/deep orange family domain, Clathrin/VPS domain, Helo_like_N domain, Ring finger/U-box domain, and sgRNAs are shown in green, blue, purple, orange, and black, respectively. Red dashes represent the six SNPs that lead to amino acid changes identified via wild type and wp tsl mutant strains' deep orange genomic sequence comparison. B Ccdor gene SNPs Sanger sequenced in Benakeion Volos FF26 (hence Benakeion) (wild-type), Seibersdorf FF26 (hence SEIB) (wild-type), VIENNA 8 2010 FF26 (hence VIENNA 8) (GSS), D53-3–28 FF21 (hence D53 tsl) (mutant) and wp tsl FF21 (hence wp tsl) (mutant) strains using as reference Ccap 3.2.1 (accession GCA_905071925.1) genome