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Fig. 3 | BMC Biotechnology

Fig. 3

From: Research on the targeted improvement of the yield of a new VB12-producing strain, Ensifer adhaerens S305, based on genomic and transcriptomic analysis

Fig. 3

Sequence alignment analysis of the VB12 synthesis pathway gene and preliminary determination of the VB12 yield in the S305 strain. A Heatmap for comparison of gene sequence similarity between Ensifer adhaerens S305 (GenBank assembly accession GCA_029439625.1) and other strains with VB12 synthesis-related genes. The horizontal axis shows the strains E. adhaerens Casida A (GenBank assembly accession GCA_029674665.1), E. adhaerens Corn53 (GenBank assembly accession GCA_009883655.1), Pseudomonas denitrificans SC 510 (European Patent Number EP-0516647-B1), Sinorhizobium fredii NGR234 (NC_012587.1), E. adhaerens OV14 (GenBank assembly accession GCA_000583045.1), Sinorhizobium fredii CCBAU 45436 (GenBank assembly accession GCA_003100575.1), and Sinorhizobium meliloti 1021 (GenBank assembly accession GCA_000006965.1) from left to right, and the vertical axis shows the similarity of VB12 synthesis-related gene sequences (0–100%) from top to bottom. The "white box" indicates that the gene sequence for the strain has not been found. B VB12 production by the S305 and Casida A strains in liquid shake flask culture with different fermentation media

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