Table 1 Optimisation of antibody concentration
From: A magnetic solid phase chemiluminescent immunoassay for quantification of Cystatin C in human serum
Anti-Cys-C-FITC (μg/mL) | 0.5 | 0.8 | 2.0 | ||||||
|---|---|---|---|---|---|---|---|---|---|
Anti-Cys-C-AP (μg/mL) | 0.5 | 0.8 | 2.0 | 0.5 | 0.8 | 2.0 | 0.5 | 0.8 | 2.0 |
Standard 1 (0.0 μg/mL) | 3228 | 3206 | 3185 | 3674 | 4143 | 6254 | 3260 | 4155 | 3911 |
Standard 2 (0.2 μg/mL) | 35,958 | 36,370 | 38,082 | 155,108 | 159,117 | 172,135 | 68,499 | 71,469 | 76,290 |
Standard 3 (6.0 μg/mL) | 395,290 | 428,691 | 417,072 | 2,396,293 | 2,606,007 | 2,649,074 | 813,664 | 1,043,755 | 1,082,789 |