Fig. 8

 H. polymorpha fed-batch cultivation using 48-well microtiter plates with black polymer rings containing glucose crystals. Cultivation was performed in n replicates (given in Fig. 8B) with H. polymorpha in Syn6-MES medium using two microtiter plates, one with DOT and pH optodes (MTP-R48-B and MTP-R48-BOH). One plate was used for measurement in the commercial BioLector, the other one for measurement in the µRAMOS-device. The vertical grey dotted line marks the beginning of the fed-batch phase. Polymer rings (both plates): polymer rings with black dye and glucose crystals, d_inner = 8 mm, Z_height = 3 mm, H_position = 0 mm, 1.5 and 3.5 mm. Experimental parameters: initial pH = 6.0, V_L = 0.8 mL, T = 30 °C, n = 1000 rpm, d₀ = 3 mm, OD_{600 nm, start} = 0.5. Commercial BioLector settings: Gain 15, λ_ex,em = 620 nm for scattered light, λ_ex = 520 nm and λ_em = 600 nm for dissolved oxygen tension (DOT), λ_ex = 470 nm and λ_em = 525 nm for pH, λ_ex = 488 nm and λ_em = 520 nm for green fluorescence, glass fibre in standard position (at for e.g., well A1 at X = 14 mm and Y = 75 mm, offset = 0 mm, Figure S3A).