Fig. 7

E. coli fed-batch cultivation using 48-well microtiter plates with black polymer rings containing glucose crystals. Cultivation was performed in n replicates (given in Fig. 7B) with E. coli in Wilms-MOPS-medium using two microtiter plates, one with DOT and pH optodes (MTP-R48-B and MTP-R48-BOH). One plate was used for measurement in the BioLector, the other one for measurement in the µRAMOS-device. The first vertical grey dashed line marks the end of the batch phase. The second vertical grey line marks the beginning of the fed-batch phase with glucose release by the feed rings. Polymer rings (both plates): polymer rings with black dye and glucose crystals, d_inner = 8 mm, Z_height = 3 mm, H_position = 0 mm, 1.5 and 3.5 mm. Experimental parameters: initial pH = 7.5, V_L = 0.8 mL, T = 37 °C, n = 1000 rpm, d₀ = 3 mm, OD_{600 nm, start} = 0.5. Commercial BioLector settings: Gain 15, λ_ex,em = 620 nm for scattered light, λ_ex = 520 nm and λ_em = 600 nm for DOT, λ_ex = 470 nm and λ_em = 525 nm for pH, λ_ex = 450 nm and λ_em = 495 nm for FbFP fluorescence, glass fibre in standard position (offset = 0 mm, Fig. S3A).