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Fig. 5 | BMC Biotechnology

Fig. 5

From: Black glucose-releasing silicon elastomer rings for fed-batch operation allow measurement of the oxygen transfer rate from the top and optical signals from the bottom for each well of a microtiter plate

Fig. 5

Influence of differently coloured polymer rings on scattered light measured in a commercial BioLector device. Polymer rings: (A) white polymer rings with glucose crystals (Fig. 2B) and (B) rings without glucose crystals sprayed with black lacquer (Fig. 2C), both with dinner = 8 mm, Zheight = 3 mm, Hposition = 0.0 mm, 1.5 mm, 3.5 and 5.5 mm. As a control, wells without polymer rings were evaluated. Experimental parameters: VL = 0 mL, T = 37 °C, n = 1000 rpm, d0 = 3vmm. Commercial BioLector settings: Gain 15, λex, em = 620 nm, three replicates measured three times, position of the X-Y-positioning device (glass fibre) was varied on the X-axis (Fig. S3) with a positive offset of 0 to 6.5 mm relative to the standard position.

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