Table 2 Half maximal effective concentration (EC50) and limit of detection (LOD) values of antibodies that were determined from the titration curves of sandwich ELISA
From: Generation of a recombinant antibody for sensitive detection of Pseudomonas aeruginosa
Antigen | Capturing antibody | Detecting antibody | EC50 (CFU/mL) | LOD (CFU/mL) |
|---|---|---|---|---|
UCBPP-PA14 | Monoclonal antibody (mAb) | Polyclonal antibody (pAb) | 1.43 ± 0.44 × 106 | 1.39 × 105 |
UCBPP-PA14 | pAb | mAb | 4.62 ± 2.80 × 106 | 8.50 × 102 |
UCBPP-PA14 | Recombinant antibody (rAb) | pAb | 4.10 ± 1.68 × 106 | 3.34 × 104 |
UCBPP-PA14 | rAb | mAb | 2.00 ± 0.06 × 106 | 7.46 × 104 |
ATCC 27,853 | mAb | pAb | 4.10 ± 0.39 × 104 | 2.04 × 103 |
ATCC 27,853 | pAb | mAb | 4.56 ± 1.11 × 105 | 5.78 × 103 |
ATCC 27,853 | rAb | pAb | 7.01 ± 0.79 × 104 | 7.85 × 103 |
ATCC 27,853 | rAb | mAb | 8.21 ± 0.95 × 105 | 6.30 × 104 |
ATCC BAA-2108 | mAb | pAb | 9.19 ± 0.56 × 104 | 3.79 × 103 |
ATCC BAA-2108 | pAb | mAb | 1.20 ± 0.04 × 105 | 7.70 × 102 |
ATCC BAA-2108 | rAb | pAb | 1.39 ± 0.22 × 104 | 2.30 × 102 |
ATCC BAA-2108 | rAb | mAb | 1.63 ± 0.17 × 105 | 1.84 × 104 |