Table 3 Conversions and product ee-values for the reduction of rac-2-phenylpropanal using a lyophilized and rehydrated whole-cell biocatalyst. The effects of catalyst loading, substrate concentration, coenzyme concentration and the addition of HBC (2-hydroxypropyl-β-cyclodextrin) were investigated.a,b
From: Reductive enzymatic dynamic kinetic resolution affording 115 g/L (S)-2-phenylpropanol
Catalyst loading (gCDW/L) | rac-2-Phenylpropanal (M) | NAD+ (mM) | HBC (mM) | Phenylpropanol (mM) | ee of (S)-Phenylpropanol (%) |
|---|---|---|---|---|---|
20 | 1 | 3 | 0 | 234 ± 16 | 95.1 ± 0.0 |
20 | 1 | 3 | 38 | 401 ± 10 | 94.1 ± 0.0 |
20 | 1 | 3 | 75 | 406 ± 2 | 93.3 ± 0.1 |
40 | 1 | 3 | 0 | 506 ± 14 | 92.2 ± 0.0 |
40 | 1 | 6 | 0 | 619 ± 24c | 93.3 ± 1.1c |
40 | 1 M fed-batch | 6 | 0 | 611 ± 12 | 94.0 ± 0.1 |
40 | 1 | 8 | 0 | 662 ± 9 | 94.1 ± 0.1 |
40 | 1 | 10 | 0 | 843 ± 31 | 93.1 ± 0.2 |
40 | 1 | 12 | 0 | 839 ± 36 | 92.9 ± 0.1 |
40 | 1 | 14 | 0 | 765 ± 31 | 92.3 ± 0.1 |
40 | 1 | 6 | 38 | 634 ± 8 | 94.3 ± 0.2 |
40 | 1 | 6 | 75 | 598 ± 7 | 92.9 ± 0.2 |
40 | 2 | 6 | 0 | 571 ± 10 | 95.4 ± 0.1 |
40 | 2 | 6 | 38 | 633 ± 2 | 93.4 ± 0.5 |
40 | 2 | 6 | 75 | 637 ± 22 | 92.2 ± 0.2 |