Table 2 Kinetic parameters of phenolic acid substrates of P-WT and mutants

P-C

V_max (IU/mg)

193.80 ± 2.38

97.73 ± 5.86

65.66 ± 4.79

12.36 ± 0.79

K_m (mmol/L)

2.05 ± 0.29

1.03 ± 0.38

3.04 ± 0.69

1.31 ± 0.03

k_cat/K_m (mmol/L/s)

13.41 ± 0.45

28.15 ± 0.28

23.24 ± 0.52

40.87 ± 0.28

P-N

V_max (IU/mg)

683.10 ± 7.38

208.50 ± 8.38

143.80 ± 5.89

10.36 ± 0.34

K_m (mmol/L)

7.02 ± 0.61

2.14 ± 0.83

1.48 ± 0.73

1.06 ± 0.42

k_cat/K_m (mmol/L/s)

14.53 ± 0.53

33.57 ± 0.49

37.57 ± 0.62

28.44 ± 0.53

P-m1

V_max (IU/mg)

17.26 ± 0.38

19.07 ± 8.39

124.60 ± 3.86

16.66 ± 0.78

K_m (mmol/L)

1.88 ± 0.53

2.08 ± 0.28

1.36 ± 0.28

1.82 ± 0.38

K_cat/K_m (mmol/L/s)

152.38 ± 1.12

28.75 ± 0.39

32.14 ± 0.52

19.17 ± 0.19

P-m2

V_max (IU/mg)

136.20 ± 8.36

111.90 ± 10.91

72.64 ± 6.89

5.08 ± 0.58

K_m (mmol/L)

1.22 ± 0.26

1.00 ± 0.38

6.49 ± 0.53

4.55 ± 0.61

k_cat/K_m (mmol/L/s)

15.97 ± 0.27

36.54 ± 0.36

4.07 ± 0.64

13.24 ± 0.66

P-Nm1

V_max (IU/mg)

254.40 ± 7.38

103.20 ± 7.48

87.37 ± 8.64

5.60 ± 0.53

K_m (mmol/L)

3.52 ± 0.32

1.43 ± 0.06

1.21 ± 0.43

0.77 ± 0.64

k_cat/K_m (mmol/L/s)

11.31 ± 0.36

19.94 ± 0.73

24.72 ± 0.63

45.67 ± 0.25

P-Nm2

V_max (IU/mg)

186.30 ± 6.39

99.09 ± 0.3.83

144.20 ± 7.32

5.27 ± 0.17

K_m (mmol/L)

12.05 ± 0.27

6.4 ± 0.38

9.33 ± 0.28

3.41 ± 0.26

k_cat/K_m (mmol/L/s)

10.33 ± 0.18

14.14 ± 0.59

10.38 ± 0.97

11.69 ± 0.11

P-WT

V_max (IU/mg)

121.50 ± 7.88

22.32 ± 4.31

89.46 ± 10.35

9.22 ± 0.19

K_m (mmol/L)

4.09 ± 0.59

2.39 ± 0.58

3.10 ± 0.47

3.20 ± 0.30

k_cat/K_m (mmol/L/s)

9.74 ± 0.53

16.49 ± 0.42

9.76 ± 0.26

16.94 ± 0.58