Fig. 2

The formation of mutants for a monomer. A Schematic overview of the recombinant derivatives of P-WT. P-WT is the phenolic acid decarboxylase isolated from Bacillus amyloliquefaciens ZJH-01. P-N extended the N-terminal of P-WT. P-C extended the C-terminus of P-WT. P-m1 mutated the His92 to 92Glu. P-m2 mutated the Tyr122 to 122Leu. P-Nm1 extended the N-terminus of P-WT and mutated the His92 to 92Glu. P-Nm2 extended the N-terminus of P-WT and mutated the Tyr122 to 122Leu. We firstly measured the enzyme activity and specific activity of the N-terminal and C-terminal mutants, and found that the N-terminal had a greater influence on it than the C-terminal. So m1 and m2 combined with N-terminal but not with C-terminal. B The alignment of the amino acid sequence of the phenolic acid decarboxylase enzyme isolated from different strains. The P-WT is a phenolic acid decarboxylase in this experiment. The 3NAD, 2W2A, 3NX2 were obtained from the protein database