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Fig. 4 | BMC Biotechnology

Fig. 4

From: Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA

Fig. 4

Visual color changes of developed NCL probe-AuNPs hybridization assay at different BVDV-RNA concentrations. Wells 1–6 contain 0.012, 0.124, 1.24, 12.4, 62, and 124 ng/reaction of RNA, respectively (a). Standard curve for the relationship between BVDV-RNA concentrations (ng/reaction) and absorbance ratio (A620/A525) (b) and BVDV concentrations (PFU/reaction) and absorbance ratio (A620/A525) (c). Inserts are the plot of linear correlation for RNA concentrations (15.5 to 62 ng/reaction) (b) and for BVDV concentrations (14 to 42 PFU/reaction) (c). The RNA concentrations 12.4 × 10^ (− 4) ˗12.4 × 10^ (− 6) showed absorbance ratio bellow 0.1

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BMC Biotechnology

ISSN: 1472-6750

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