Fig. 5From: The next generation of biopanning: next gen sequencing improves analysis of bacterial display librariesStrategy for filtering NGS sequences. A valid insert sequence consists of correct flanking regions, an insert within the correct reading frame (typically 45 bp), and without stop codons (a). Sequences that translate into an insert with stop codons (b), result in a frame shift (c), or that have no insert (d) are filtered into separate categoriesBack to article page