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Table 3 Primers used in this study

From: Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis

PrimersSequencesRE
F-attBCTACTGCTGCTTCACCAGTTT
R-attBGTATGCAGCGATGTTGTTACCC
F-attPGGAC CTCGAGTCCAACTCGCTTAATTGCXhoI
R-attPGGACGGATCCGCTAAAACGTCTCAGAAABamHI
F-IntGACGCCATGGCACATCATCATCATCATCATATGACTAAGAAAGTAGCAATCTATACNcoI
R-IntGCACGAGCTCTTAAGCGAGTTGGAATTTAAATATGHindIII
F-PnisAGCGAGATCTAGTCTTATAACTATACBglII
R-PnisAGCAGATATCGTGAGTGCCTCCTTATAATEcoRV
F-P170GCGACAGATCTGAACTATGAATATCBglII
R-P170GCACGATATCAACTGTTCTTTTTTAATTTTTEcoRV
F-USP45GACGGATATCATGAAAAAAAAGAEcoRV
R-USP45CTGAGTCGACGATATTTCGAGAGCSalI
F-SpkNucGCGTGATATCATGAAAAAAATATTAACGTTGGTATEcoRV
R-NucHGGCGAGCTCTTAGTGGTGATGATGGTGASacI
F-NucEVGCGTGATATCATGAAAAAAATATTAACGTTGGTATEcoRV
F-NucNICGAGCCATGGCTATGAAAAAAATATTAACGNcoI
R-NucEICGCGGAATTCTTGACCTGAATCAGCGTTGTCTTCGEcoRI
F-EmGACGGGATCCAATCAGGCTTGATCCCCAGTAAGTCBamHI
R-EmGACGAGATCTAGGATGAGGAGGCAGATTGCCTTGBglII
F-PrtP344GAGCGAATTCTGGCTCTAGAGGATCCAAGTCEcoRI
R-PrtP344TAAACTATGCGGCCGCGACAGGCTATTCTTCNotI
F-E. coli OriGGAGGGATCCGGCGTAATCATGGTCATAGBamHI
R-E. coli OriAGGCGGATCCCTGTCAGACCAAGTTTACTCBamHI
  1. RE sites are in bold