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Fig. 4 | BMC Biotechnology

Fig. 4

From: Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element (UCOE)

Fig. 4

UCOE-CORE element provides direction-dependent, robust protection against transgene repression. Schematic illustration of the SB transposon vectors carrying the UCOE-CORE element and eGFP expression profile of individual CHO-K1 clones harboring these vectors over a seven-week period of continuous culture in the absence of puromycin selection pressure with the UCOE-CORE in either the 5′-(a) or 3′- (b) orientation. Expression of eGFP was assessed by flow cytometry. Each line in the graphs represents the expression profile of a single clone over the course of the 7 weeks of continuous culture

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BMC Biotechnology

ISSN: 1472-6750

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