Table 2 Glucose, lactate, and glutamine content and pH of the ACF media 20 h after viral infection
From: Investigation of cell culture conditions for optimal foot-and-mouth disease virus production
virus | A IRN/8/2015 | NC | A IRN/8/2015 | NC | A IRN/8/2015 | NC | |||
cell density | 1 × 106 cells/mL | 2 × 106 cells/mL | 3 × 106 cells/mL | ||||||
media exchange | 100% | 30% | 30% | 100% | 30% | 30% | 100% | 30% | 30% |
pH | 7.5 ± 0.1 | 7.3 ± 0.1 | 7.1 ± 0.2 | 7.3 ± 0.1 | 7.0 ± 0.1 | 7.0 ± 0.1 | 7.2 ± 0.1 | 7.0 ± 0.3 | 6.9 ± 0.1 |
glucose (g/L) | 5.2 ± 1.2 | 3.4 ± 1.0 | 2.6 ± 0.3 | 3.7 ± 1.8 | 1.6 ± 0.4 | 1.4 ± 0.6 | 2.6 ± 1.0 | 1.1 ± 0.5 | 1.2 ± 0.6 |
lactate (mM) | 8.2 ± 0.9 | 23.0 ± 6.1 | 26.9 ± 4.6 | 16.6 ± 3.1 | 28.5 ± 4.1 | 29.2 ± 3.5 | 18.5 ± 2.6 | 25.1 ± 5.3 | 33.6 ± 4.1 |
glutamine (mM) | 2.5 ± 1.8 | 1.5 ± 1.0 | 0.4 ± 0.3 | 2.5 ± 0.9 | 0.6 ± 0.6 | 0.1 ± 0.1 | 1.6 ± 0.6 | 0.3 ± 0.3 | 0.7 ± 0.9 |
virus isolate | O SAU/18/2015 | NC | O SAU/18/2015 | NC | O SAU/18/2015 | NC | |||
cell density | 1 × 106 cells/mL | 2 × 106 cells/mL | 3 × 106 cells/mL | ||||||
media exchange | 100% | 30% | 30% | 100% | 30% | 30% | 100% | 30% | 30% |
pH | 7.4 ± 0.0 | 7.4 ± 0.0 | 7.1 ± 0.0 | 7.4 ± 0.0 | 7.2 ± 0.2 | 7.0 ± 0.2 | 7.2 ± 0.20 | 6.9 ± 0.2 | 6.9 ± 0.2 |
glucose (g/L) | 7.0 ± 1.6 | 5.2 ± 1.0 | 3.5 ± 1.0 | 5.1 ± 0.7 | 3.1 ± 1.2 | 2.1 ± 0.8 | 4.1 ± 0.3 | 1.5 ± 0.9 | 1.6 ± 0.5 |
lactate (mM) | 7.5 ± 4.1 | 11.5 ± 5.8 | 21.4 ± 10.7 | 14.9 ± 6.8 | 23.7 ± 11.1 | 27.9 ± 14.0 | 16.6 ± 8.4 | 29.2 ± 14.9 | 36.8 ± 19.1 |
glutamine (mM) | 4.3 ± 1.5 | 3.1 ± 0.9 | 1.3 ± 0.3 | 4.0 ± 1.3 | 1.6 ± 0.7 | 0.5 ± 0.4 | 2.6 ± 0.5 | 0.7 ± 0.3 | 0.2 ± 0.1 |