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Fig. 3 | BMC Biotechnology

Fig. 3

From: Recovery of recombinant Mycobacterium tuberculosis antigens fused with cell wall-anchoring motif (LysM) from inclusion bodies using non-denaturing reagent (N-laurylsarcosine)

Fig. 3

Solubilization and purification of ARL inclusion bodies (IBs) using varying concentrations of 0.5 1, 3 and 5% (w/v) NLS a Soluble fractions of ARL after NLS treatment. b Insoluble fractions of ARL after NLS treatment. c Ratio of soluble protein to insoluble protein of ARL obtained from IBs solubilized with varying NLS concentration. d 5% NLS-treated ARL which was diluted to 0.1% NLS initially before the purification via the Ni2+-NTA affinity chromatography

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BMC Biotechnology

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