Fig. 2

Detection of transgenic soybean plants. a Detection of CRISPR-Cas9 binary vector in Agrobacterium strain (EH105) using CRISPR specific primers where M: represents Marker or ladder and lanes 1–6 represents positive colons. b PCR analysis of BAR gene in transgenic plants of soybean, where wild type DNA (WT) and our constructed plasmid (P) were taken as negative and positive control. From 1 to 8 revealed different positive soybean plants. c Detection of NOS terminator in soybean transgenic plants. We used plasmid as positive control while (WT) represent negative, Lanes 1 to 8 indicates NOS terminator existence in soybean transformed plants. Based on these outcomes we confirmed the existence of the binary vector in soybean transgenic plants