Fig. 4

Mxr1-reprogrammed cells were induced under broad conditions. a AOX activity in different cells in response to different carbon sources. b After being cultured in BMGY (2% glycerol), cells were concentrated in the original medium, or the medium was replaced by fresh medium without residual glycerol. The cells were then induced with 0.5% methanol per day. The results represent the mean of three independent biological replicates. Two-way ANOVA and the least significant difference (LSD) test were used to determine significance (p = 0.05). c After being cultured in BMGY (2% glycerol), cells were cultured in BMGY and induced with 0.33% glycerol per day. The error bars represent the standard deviation of three biological replicates. The independent-sample t-test was used to determine significance. NS, p > 0.05; ***, p < 0.005