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Table 1 Pregnancy outputs from cloned embryos according to cell line

From: Establishment of TP53-knockout canine cells using optimized CRIPSR/Cas9 vector system for canine cancer research

Cell type

Nuclear transfer

Embryo transfer

No. of oocyte donors dogs

No. of

No. of surrogate

dogs

No. of pregnancies (%)a

Oocyte subjected to NT

Oocyte fused & transferred (%)c

Offspring born

(%)d

On Day 30

Full Termb

K9 Fetus 1 (control)

14

121

109 (90.1)

3 (2.8)e

7

3 (21.1)e

3 (21.1)e

Tp53KO#30

(1st trial)

43

491

218 (50.5)

0 (0.0)

15

0 (0.0)

0 (0.0)

Tp53KO#30

(2nd trial)

66

770

282 (42.3)

0 (0.0)

17

0 (0.0)

0 (0.0)

  1. a Pregnancy was detected using real-time ultrasonography. Pregnancy rate was calculated based on the number of pregnancies observed out of the total number of surrogates that were transferred with reconstructed embryos (fused oocytes)
  2. b Full term pregnancy refer to pregnancy that successfully produced live offspring
  3. c Fusion rate was calculated based on the number of fused oocytes out of the total oocytes that were subjected to nuclear transfer (NT)
  4. d The offspring born were calculated based on the total number of reconstructed embryo transferred
  5. e Superscripts in different rows in the same column differ significantly (P < 0.05)
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BMC Biotechnology

ISSN: 1472-6750

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