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Fig. 2 | BMC Biotechnology

Fig. 2

From: Development of luciferase-linked antibody capture assay based on luciferase immunoprecipitation systems for antibody detection of porcine reproductive and respiratory syndrome virus

Fig. 2

Expression and characterization of Renilla luciferase-fused PRRSV-N (N-Rluc) and nsp1α (nsp1α-Rluc). a HEK293T cells were transfected with the indicated plasmids for 48 h followed by detection of expressed recombinant proteins bound to anti-Renilla luciferase-conjugated polyclonal antibodies or anti-PRRSV-N monoclonal antibody 6D10 via Western blotting; b The luciferase activities of recombinant N-Rluc and nsp1α-Rluc in cell lysates were evaluated upon addition of Renilla luciferase substrate and compared to lysates of cells transfected with pGL4.74-hRL-TK. c BHK-21 cells transfected with indicated plasmids probed with either PRRSV-SD16 convalescent pig serum (Green channel) or anti-Renilla luciferase polyclonal antibodies (Red channel) by immunofluorescence assay

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BMC Biotechnology

ISSN: 1472-6750

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