Fig. 3
From: Infectivity of adeno-associated virus serotypes in mouse testis
Tropism of Phosphodegron mutants of AAV2 and AAV9. AAV2 TM (triple mutant carrying S489A, T251A and K532R mutations) and AAV9 S499A were injected into 4 weeks old males. 1 X 109 vgs / testis; n = 3 animals. a Testes wholemount showing live GFP expression. b Confocal microscopic analysis comparing wildtype AAV2 with that of AAV2 TM. Sal4 and PLZF are spermatogonial stem cell markers. The images show the distribution of GFP+ cells in the seminiferous tubules in AAV2 TM injected testis. Yellow arrowheads highlight Sal4+ SSCs, which are GFP negative. c Immunostained cryosections of testis comparing wildtype AAV9 with that of AAV9 S499A mutant transduction (See Fig. S1C for quantitation). Scale bar 50 and 100 μm, for low and high magnification images, respectively