Characterization of a strong and constitutive promoter from the Arabidopsis serine carboxypeptidase-like gene AtSCPL30 as a potential tool for crop transgenic breeding

Table 1 PCR primers used in the present study

Name	Forward (5′-3′)	Reverse (5′-3′)
P _AtSCPL30FR	ctaatctcaatgtttccgcctttc	cacatgaaaccatttctactaatat
PD1FR	atcggatccctaatctcaatgtttccgc	attccatggttgaggctaggttttagtag
PD2FR	atcggatccaagggaactacgcaaga	attccatggttgaggctaggttttagtag
PD3FR	atcggatccctgctttcgatcatttc	attccatggttgaggctaggttttagtag
PD4FR	atcggatccatggagttagagtttac	attccatggttgaggctaggttttagtag
PD5FR	atcggatcctaagtcgatcaatccgc	attccatggttgaggctaggttttagtag
PD6FR	atcggatccccgaatccacgaaac	attccatggttgaggctaggttttagtag
PD7FR	atcggatccataggcaaccgtggact	attccatggttgaggctaggttttagtag
PD8FR	atcggatccttatccctgtaaatcc	attccatggttgaggctaggttttagtag
PD9FR	atcggatccgctaccaatttaccaca	attccatggttgaggctaggttttagtag
P _CaMV35SFR	aatggatccaagtctcaatagcccttt	tgagaattccgtattggctagagcagc
PD7-8FR	atcggatccataggcaaccgtggact	agcctgcagattcgaatgaattcgtatat
PD8–9 FR	atcggatccttatccctgtaaatcct	aagctgcagggtagctttagtttattga
PD7-9FR	atcggatccataggcaaccgtggact	aagctgcagggtagctttagtttattga
HPTFR	cgtctgctgctccatacaa	tgtcctgcgggtaaatagc
P _ZmUbi-1FR	gataagcttctgcagaagtaacaccaaacaatcg	agcggatccgcatgcctgcagtgcaactttatat

The underlined sites are the sites for the digestion of restriction enzymes BamHI. The underlined italicized sites are the sites for the digestion of restriction enzymes NcoI. The sites in bold are the sitess for the digestion of restriction enzymes PstI. The italicized site is the site for the digestion of restriction enzymes HindШ

ISSN: 1472-6750