Fig. 2
SDS-PAGE analysis of small-scale expression a, large-scale expression and purification steps of R101 protein b, and western blot analysis of R101 protein* c, d, e. Lane 1, non-induced bacteria; lanes 2–6, expression levels of different single colonies; lane 7, non-induced bacteria; lane 8, total bacterial proteins after sonication; lane 9, flow-through fraction from affinity chromatography; lane 10, supernatant from the homogenate; lane 11, eluates washed with 300 mM imidazole in affinity chromatography; lane 12, eluates washed with 200 mM NaCl in DEAE chromatography; lane 13, non-induced bacteria; and lane 14, purified R101 protein *C. Mouse anti-Trx monoclonal antibody as primary antibody; *D. Mouse anti-His monoclonal antibody as primary antibody; and *E. Mouse anti-PLA2R monoclonal antibody as primary antibody. All of the secondary antibodies were goat anti-mouse IgG-HRP conjugates.