Fig. 1

Domains of the R101 gene in the pET43.1 expression vector a and R101 fragment b. Expression vector c and restriction endonuclease analysis of the expression plasmid d, (NcoI and XhoI). Agarose gel electrophoresis showed the R101 fragment b and expression vector c obtained by dealing pMD-19 T-R101 plasmid and pET43.1a vector with NcoI – XhoI, respectively