Fig. 7From: Heterologous expression and biochemical characterization of a GHF9 endoglucanase from the termite Reticulitermes speratus in Pichia pastorisEffects of divalent metal ions on RsEGm activity. The effects of metal ions on the catalytic activity of RsEGm were determined by adding 1 mM of various divalent metals to the standard enzyme assay system (pH 5.0, 37°C for 15 min). The activity at pH 5.0 and 37°C (6.7 U/mg) in the absence of divalent metal ions (control) was taken as 100%, and the percentage of the activity in the presence of different divalent metal ions against the control was calculated. Values are expressed as the means of three replicates ± standard deviationBack to article page