Purification step | Total protein (mg) | Total Activity (Units) | Specificity (Units/mg) | Enzyme recovery (%) | Purification fold |
---|---|---|---|---|---|
a | |||||
Crude Extract | 29150 ± 1457 | 12748 ± 637 | 0.44 ± 0.02 | n.a | n.a |
Thermo-precipitate | 8352 ± 250 | 10793 ± 324 | 1.29 ± 0.04 | 84.67 ± 2.54 | 3.01 ± 0.09 |
Retentate 100 kDa | 4000 ± 80 | 751 ± 15 | 0.19 ± 0.004 | 5.89 ± 0.12 | n.a |
Permeate 100 kDa | 4349 ± 174 | 10045 ± 402 | 2.31 ± 0.10 | 78.80 ± 3.10 | 5.37 ± 0.20 |
Retentate 5 kDa | 3000 ± 60 | 9843 ± 196 | 3.28 ± 0.06 | 77.21 ± 1.54 | 7.63 ± 0.15 |
Permeate 5 kDa | 1289 ± 26 | 201 ± 4 | 0.15 ± 0.003 | 1.50 ± 0.03 | 0.34 ± 0.007 |
b | |||||
Crude Extract | 43560 ± 1306 | 58355 ± 1750 | 1.34 ± 0.04 | n.a | n.a |
Thermo-precipitate | 12273 ± 613 | 49421 ± 2471 | 4.06 ± 0.20 | 84.69 ± 4.23 | 3.03 ± 0.15 |
Retentate 100 kDa | 7002 ± 175 | 3754 ± 94 | 0.53 ± 0.01 | 6.43 ± 0.16 | 0.40 ± 0.10 |
Permeate 100 kDa | 5201 ± 104 | 45661 ± 913 | 8.80 ± 0.18 | 78.25 ± 1.56 | 6.70 ± 0.13 |
Retentate 5 kDa | 2663 ± 53 | 45133 ± 902 | 17.00 ± 0.34 | 77.34 ± 1.54 | 13.10 ± 0.26 |
Permeate 5 kDa | 2520 ± 25 | 480 ± 4.8 | 0.19 ± 0.002 | 0.80 ± 0.008 | 0.15 ± 0.0015 |