Fig. 2From: Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equalFlow chart summarising differences in the protocols, time requirements and prices of the different RNA purification methods. RNA was isolated using five methods that were either phenol based (TRIzol), column-based (Isolate II and Norgen total), or combined phenol and column-based methods (miRNeasy and miRVana). $ < 5, $$ = 5–10, $$$ > 10 AUD$ per sample preparation. Time requirement based on following instructions provided by manufacturer (not taking into account extra time required by the technician to perform the tasks)Back to article page