Light induced expression of β-glucosidase in Escherichia coli with autolysis of cell

BMC Biotechnology

Table 2 Purification of Bgl1A(A24S/F297Y) and Bgl-CBM from E.coli

	Steps	Total protein (mg)	Total activity (U)	Specific activity (U/mg)	Purification (Fold)	Yield (%)
Hexahistidine affinity	Cell extract	9.23	120.62	13.07	1	100%
Hexahistidine affinity	Purificated	2.64	109.89	41.52	3.17	91.1%
Cellulose affinity	Cell extract	9.65	116.88	12.11	1	100%
Cellulose affinity	Purificated	3.29	105.89	32.12	2.65	90.6%

Data present was the average of three batches. Total protein = protein concentration (mg/mL) × volume (mL). Yield = total protein (mg) × purity (%)

ISSN: 1472-6750