Fig. 6
From: Light induced expression of β-glucosidase in Escherichia coli with autolysis of cell
SDS-PAGE of Bgl1A(A24S/F297Y)-CBM expression in E. coli using light induction and adsorption onto cellulose after autolysis. M is molecular marker, lysis is supernatant of fermentation culture after cell autolysis, super and precip are supernatant and precipitate after ultrasonic fragmentation of cells that were not autolyzed. Binding on cellulose was allowed for one hour, the supernatant and precipitate were separated by centrifugation. Bgl is purified protein using hexahistidine affinity tag