Fig. 2
From: Light induced expression of β-glucosidase in Escherichia coli with autolysis of cell
Effect of initial induction cell density on recombinant Bgl1A(A24S/F297Y) synthesis in Erlenmeyer flasks. Bgl1A(A24S/F297Y) activity after light induction at different cell densities (OD 600 = 0.6, 3.0 and 6.0) and IPTG induction at OD 600 = 0.6 (a). SDS-PAGE of Bgl1A(A24S/F297Y) induced with IPTG or light at different initial induction cell density (b), M is molecular marker, blank represents BL21(DE3)-pET22b without bgl insertion, IPTG represents BL21(DE3)/pET22b-T7-bgl induced with IPTG, OD 600 = 0.6, OD 600 = 3.0 and OD 600 = 6.0 represent BL21(DE3)-pET22b–pD-bgl induced with light at different cell density gradients