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Fig. 10 | BMC Biotechnology

Fig. 10

From: Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene – methyltetrazine reactions

Fig. 10

SDS-PAGE analysis of co-immunoprecipitation experiments under non-reducing condition. Panels: a, receptor-mediated co-immunoprecipitation using hFasRECD-Fc; b, antibody-mediated co-immunoprecipitation using biotin conjugated goat anti-rabbit IgG H&L. Lanes (in both panels): M, molecular-weight size markers; 1 and 2, Avidin-MTZ alone; 3 and 4, Avidin-hFasLECD conjugate; 5 and 6, hFasLECD-TCO alone; 7 and 8, rFab’-hFasLECD conjugate (the peak 1 fraction); 9 and 10, rFab’-hFasLECD conjugate (the combined fractions of peaks 2 and 3); 11 and 12, rFab’-MTZ alone; 1, 3, 5, 7, 9 and 11, examined samples; 2, 4, 6, 8, 10 and 12, precipitated materials (IP)

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BMC Biotechnology

ISSN: 1472-6750

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