Improved n-butanol production via co-expression of membrane-targeted tilapia metallothionein and the clostridial metabolic pathway in Escherichia coli

Table 2 Engineering strategies to improve butanol tolerance and production in E. coli

Butanol tolerance				Butanol production
	Strategy	Tolerance (v/v)	Reference	Strategy	Production (mg/l)	Reference
1	Overexpression of ompC-tmt	From 1.50 to 2.00%	[28]	thil, crt, bcd, etfA, etfB, hbd, adhe, ompC-tmt	320 mg/l	This work
2	N.D.	N.D.	N.D.	atoB, crt, bcd, etfAB, hbd, adhe, ΔadhE, ΔldhA, ΔfrdBC, Δfnr, Δpta	552 mg/l	[5]
3	N.D.	N.D.	N.D.	thil, hbd, crt, bcd, etfA, etfB, adhe, high cell density	1,200 mg/l	[6]
4	Overexpression of ATF, fabD, feoA and srpABC	From 1.50 to 2.00%	[38]	N.D.	N.D.	N.D.
5	double disruptions of proV and marR Mutation of lon	From 1.00 to 2.00%	[37]	N.D.	N.D.	N.D.
6	Overexpression of groESL	From 0.75 to 1.00%	[34]	N.D.	N.D.	N.D.
7	Mutation of cyclic AMP receptor protein (CRP)	From 0.80 to 1.20%	[33]	N.D.	N.D.	N.D.

ISSN: 1472-6750