Fig. 5

Effects of temperature, substrate and pH on growth and de-epoxidation activities of DX100 under aerobic conditions. a: OD_600nm values show relative growth performance of DX100 in different broth media. b: Bacterial de-epoxidation activity (DON to dE-DON transformation) in different broth media. c: OD_600nm values demonstrate the effects of temperatures on the growth capacity of DX100 in MSB. d: Bacterial DON de-epoxidation in μg/mL under different temperatures in MSB. e: OD_600nm values show growth behavior of DX100 at different pHs in MSB. f: Bacterial de-epoxidation (DON to dE-DON transformation) in μg/mL in different pHs. The activity was measured by quantifying de-epoxidation activity divided by microbial growth (OD₆₀₀ value) under different growth conditions. Results are the mean of five replicate observations, and bars shown are ± standard errors of the means. The averages of ± standard errors for different growth conditions are presented in the graph. Treatments labeled with the same letter are not significantly different, according to Tukey’s HSD test (p < 0.05). Media: NB = nutrient broth, 1/NB = 1/10 strength NB, MM = Minimal medium, MS-DON = six mineral salts having 200 μg/mL DON. dE-DON = de-epoxy deoxynivalenol, MSB = six mineral salts + 0.5% Bacto Peptone, MSC = six mineral salts broth + 0.3% Bacto Peptone, MSY = six mineral salts with 0.5% yeast extract, S.E = standard error, SE = soil extract, PDB = potato dextrose broth and LB = Luria Bertani