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Fig. 2 | BMC Biotechnology

Fig. 2

From: Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system

Fig. 2

Effect of SCF and LIF on growth of cBCs in E8 medium. Cell number (a) and cell viability (b) were measured after treatment for the indicated times with SCF, LIF or both. Values are the mean ± SEM (n = 5). c The expression levels of the pluripotency genes PouV, Nanog and Sox2 were measured by qRT-PCR after growth factor treatment for 24 h. Values are the mean ± SEM (n = 3). Data are representative of results in three independent experiments, and each condition is normalized to β-actin abundance. *, p < 0.05; **, p < 0.01

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BMC Biotechnology

ISSN: 1472-6750

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