Table 3 The targeting cassette vectors
From: Sequential and counter-selectable cassettes for fission yeast
Name | Promoter/gene/terminator | Selection/counter |
|---|---|---|
pFA6a-kanMX6 | Ptef/kan R /Ttef | G418 |
pFA6a-neoCV | Pcmv/neo R /Tsv40 | G418 |
pFA6a-zeoCV | Pcmv/zeo R /Tsv40 | Zeocin |
pFA6a-hygMX6 | Ptef/hph/Ttef | G418 |
pFA6a-hygMV | Ptef/hph/Tsv40 | Hygromycin B |
pFA6a-hygML | Ptef/hph/Tscleu2 | Hygromycin B |
pFA6a-natMX6 | Ptef/nat R /Ttef | ClonNat |
pFA6a-natCX | Pcmv/nat R /Ttef | ClonNat |
pFA6a-TKnatCX | Pcmv/TK-nat R /Ttef | ClonNat/FdU |
pFA6a-TKnatAX | Padh/TK-nat R /Ttef | ClonNat/FdU |
pFA6a-TKanAX | Padh/TK-kan R /Ttef | G418/FdU + HU |
pFA6a-HyTKAX | Padh/hph-TK/Ttef | Hygromycin B/FdU |
pFA6a-FTKnatAX | lox71-Padh/TK-nat R /Ttef-lox66 | ClonNat/FdU |
pFA6a-FTKanAX | lox71-Padh/TK-kan R /Ttef-lox66 | G418/FdU + HU |
pFA6a-FHyTKAX | lox71-Padh/hph-TK/Ttef-lox66 | Hygromycin B/FdU |