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Fig. 5 | BMC Biotechnology

Fig. 5

From: Evaluating the use of a CpG free promoter for long-term recombinant protein expression stability in Chinese hamster ovary cells

Fig. 5

Comparing DNA methylation and histone modifications for unstable and stable clones for CpGfree and CpGrich promoters by ChIP assays. DNA was extracted from samples of two unstable clones and one stable clone for each promoter and immunoprecipitation performed using four different antibodies: (a) anti-5-mC, (b) anti-H3K9Ac, (c) anti-H3K9Me3 and (d) anti-HP1. Enrichment was measured by real-time quantitative PCR. Black and grey bars represent data for samples from week 0 and from week 8 respectively. Results were normalized to their week 0 results and each bar represents triplicate measurements of two separate sets of experiments

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BMC Biotechnology

ISSN: 1472-6750

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