Table 1 Conditions for protoplast isolation from various Phaseolus vulgaris tissues

Leaf

ES-I

1.50 % (w/v) cellulase R10, 0.37 % (w/v) macerozyme R10

30 min

N/A

4–5 h

3 × 10⁵

cells g^-1ml^-1 FW

Flower petal

ES-II

1.50 % (w/v) cellulase R10, 0.37 % (w/v) macerozyme R10, 30 U pectinase

30 min

N/A

8–10 h

2 × 10⁵

cells g^-1ml^-1 FW

Hypocotyl & root

ES-III

2.0 % (w/v) cellulase R10, 0.3 % (w/v) macerozyme R10, 4.0 % (w/v) hemicellulase

N/A

4 h

16–18 h

2 × 10⁵

cells g^-1ml^-1 FW

Nodule

ES-IV

1.0 % (w/v) cellulase R10, 0.3 % (w/v) macerozyme R10, 1.0 % (w/v) hemicellulase, 30 U of pectinase

N/A

4 h

16–18 h

1 × 10⁵

cells g^-1ml^-1 FW