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Fig. 1 | BMC Biotechnology

Fig. 1

From: Depleting extracellular vesicles from fetal bovine serum alters proliferation and differentiation of skeletal muscle cells in vitro

Fig. 1

Fetal bovine serum (FBS) contain exosome-like vesicles. FBS-derived vesicles were pellet at 100,000 g for 18 h, resuspended in PBS and re-pelleted at 100,000 g for 70 min. a FBS-EV size distributions measured with Nanosight. b Immuno-blotting for enriched exosomal proteins in FBS (1); C2C12 released exosomes were used as positive controls (2). TSG101 = Tumor susceptibility gene 101, CD81 = Cluster of Differentiation 81 (tetraspanin). c TEM images of purified FBS-EVs. Nanovesicles are labeled with anti-CD81 gold particles to confirm their exosomal origin

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BMC Biotechnology

ISSN: 1472-6750

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